Evaluate the Point Spread Function (PSF) of your objective

 

Program is designed to analyze large number of beads on a FOV

Provides heat map showing planarity detects, spherical and other aberrations.

http://www.knoplab.de/psfj/

http://www.nature.com/nmeth/journal/v11/n10/full/nmeth.3102.html

Credit: Patrick Theer, Cyril Mongis, & Michael Knop

 

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